P - Pen tool. for making art.

E - Eraser tool.

I - Pipette, for selecting a color on the canvas. Switches to Pen tool after being used.

W - Magic Wand, select groups of neighboring pixels.

Ctrl + Z, Ctrl + Y - To undo and redo actions.

G - Gradient Tool, creates gradients based on highlighted pixels.

D - Dithering Pen, make pixels darker or lighter.

Alt + E - Eraser Pen, basically just like the pen tool.

Alt + B - Brush Tool, which can be used to quickly lay out patterns of pixels.

Alt + S - Shape Tool, for quickly creating pixel circles, squares, and more advanced shapes.

Alt + G - Grid Tool, displays a grid (can be customized in the settings.)

T - Text Tool, for putting down text.

M - Move tool, for moving a selection of pixels around.

L - Opens the Layers Toolbar, for managing art layers.

A - Opens the Animation Toolbar, for managing animation frames.

Shift + C - Clone tool, clone a selection or animation frame.

S - Select Rectangle tool. Click and drag a portion of your pixel art to move, rotate, or delete it.

B - Paint bucket, cover the canvas or an enclosed portion with one color.

R - Color Replace, replaces all pixels of the same color.